Setting the Stage: Where Have We Been, Where Are We Now, Where Are We Headed
8:00 – 8:30 AM Nathan Bryan – Welcome, Introduction, Meeting Expectations
8:30 – 9:15 AM Lou Ignarro – Nobel Prize Discoveries
9:15 – 10:00 AM Jonathan Stamler – General Overview of the NO Field (SWOT Analysis)
10:00 – 10:45 AM Martin Feelisch – Nitric Oxide: Where Does it Go, What Does it Become and How Does it Signal?
10:45 – 11:00 AM Meeting sponsors introduction – Patricia Therriault, AirgasTherapeutics, Inc
Nitric Oxide Production Pathways
11:00 – 11:30 AM Dennis Stuehr – Nitric Oxide Synthase and sGC Activation
11:30 – 12:00 PM Jose Tanus-Santos – From Stomach to Vasculature: How Gastric S-Nitrosothiol Formation Shapes the Cardiovascular Actions of Oral Nitrite and Nitrate
12:00 – 1:00 PM Oral abstracts (6 presentations 10 minutes each)
12:00 – 12:10 PM – Barbara Piknova - Nitric oxide availability - age-related changes. Animal study
12:10 – 12:20 PM – Bruce King - Differential Reactivity of Bacillithiol, Mycothiol and Glutathione with Nitroxyl (HNO): Structural Determinants and Potential Biological Implications
12:20 – 12:30 PM – Yuri Kato - Maintaining mitochondrial quality control prevents Heart Failure with Preserved Ejection Fraction
12:30 – 12:40 PM – Vinod Kumar – In situ activation of nitric oxide via intracarotid sodiumnitroprusside in sub-acute ischemic stroke with double plasma rich protein forphysiological recovery via 10,000 fold effect – RCT IIB in 1265 cases
12:40 – 12:50 PM – Taiming Liu - Heme-NO dilates arteries via mobilizing NO moieties froman intracellular NO store in vascular wall
12:50 – 1:00 PM - Mahmoud H. Elbatreek - A Novel Translational Multi-Organ Model of Cardiometabolic HFpEF Involving Genetic eNOS Ablation and High-Fat Diet
Cardiovascular Disease and NO Signaling
1:00 – 1:45 PM Joseph Loscalzo – NO, Endothelial Phenotype, and Ischemia-Reperfusion Injury
1:45 – 2:30 PM Wally Koch – Role of GRK2 S-Nitrosylation during Cardiac Injury and Repair
2:30 – 3:00 PM Kathy Maglioto – Plethysmography for Endothelial Dysfunction
3:00 – 3:15 PM Meeting Sponsors Introduction
3:15 – 4:00 PM Amrita Ahluwalia – Inorganic Nitrate as a Treatment forHypertension
04:00 PM – Closing Remarks
Asthma, Infections and Respiratory Medicine
8:00 – 8:30 AM Serpil Eruzum – The Role of Nitric Oxide in Adaptive Hypoxia
8:30 – 9:15 AM Ben Gaston – GSNORi as a Therapeutic Target for Asthma
9:15 – 10:00 AM Mark Schoenfisch – Nitric oxide-releasing prodrug for the treatment of chronic respiratory infections
10:00– 10:30 AM Lorenzo Berra – Inhaled Nitric Oxide and Respiratory Failure
10:30 – 10:45 AM Meeting sponsor introductions – Bryan Therapeutics, Inc.
Novel Therapies and Biomedical Engineering
10:45 – 11:15 AM Binglan Yu – Novel Indications and Delivery Devices for NO Therapy
11:15 – 11:45 PM Shawn Green – Prebiotic Nitrate: Shift the OralMicrobiome
11:45 – 12:00 Sponsor introduction – Thomas Kutz, Noxygen Theapeutics, Inc.
Nitric Oxide Biological Chemistry
12:00 – 12:45 PM Elizabeth Boon – HNOX Sensing in Bacteria
12:45 – 1:15 PM Nick LeBrun – NO Reaction with FeS Derepresses GenesImpacting Microbial Survival
1:15 – 2:00 pm Daniel Kim-Shapiro – Update on NO-Heme Chemistry and Signaling
2:00 – 3:40 PM Late breaking abstracts (10 min talks)
2:00 - 2:10 PM - Gianmarco Matrullo - GSNOR Downregulation EnhancesRhabdomyosarcoma Proliferation Through CDK4-Mediated Retino blastoma Hyperphosphorylation
2:10 – 2:20 PM - Motohiro Nishida - Supersulfide metabolism regulating cardiac maturation and robustness
2:20 – 2:30 PM - Thilini Karunarathna - Rapid Ascorbate-Catalyzed Reductive Nitrosylation of Myoglobin
2:30 – 2:40 PM - Seiryo Ogata - A Novel Catalytic Mechanism for Cyclo-Octasulfur (S8)Biosynthesis Mediated by NOX and NOS Enzymes
2:40 – 2:50 PM - Chiara Pecorari - Crosstalk between S-nitrosylation and glycation definesa novel metabolic vulnerability in liver and renal cancers
2:50 – 3:00 PM - Kelsey O’Brien - Sodium Nitrite Improves Perfusion, Oxygenation and Drug Delivery to Hypoxic Tumors
3:00 – 3:10 PM - Michael Whitt - Noninvasive Determination of Beetroot Nitrate Supplements’ Impact on Endothelial Response via Hyperemic Arterial Compliance Measurements
3:10 – 3:20 PM - Ramesh Chennupati - Red blood cells from chronic kidney disease patients promote vascular smooth muscle and endothelial dysfunction in resistance arteries
3:20 – 3:30 PM - Zohreh Safari - O2 Responsive ‘Opening’ PIEZO1 by the Motor Protein NMIIA Governs RBC eNOS Activity During Circulatory Transit
3:30 – 3:40 PM – Malcolm Kahn – Therapeutic Solutions of Nitric Oxide in Wound Care
Drug Development
3:45 PM – 4:30 PM Zhiqiang An, Ph.D. – Texas Therapeutics Institute
4:30 PM – Questions and Answers
NO Production and Signaling in Diabetes, Renal, and Metabolic Disease
8:00 – 8:45 AM John Corbett – How Nitric oxide induces "metabolic suspended animation" to protect islets
8:45 – 9:30 AM Jimmy Chang – Nitric Oxide in Urea Cycle Disorders
9:30 – 10:15 AM Erika Palmieri – Nitric Oxide Rewiring of the Immunometabolic Niche
10:15 – 10:45 Sponsor introduction – Mission Peak Health - Thorsten Meier
Cancer and Nitric Oxide
10:45 – 11:15 AM Tim Billiar – NOS Inhibitors: Indication, Trial Design, Outcomes
11:15 – 12:00 PM Dave Wink - Spatial Geography of NOS2 and COX2 in theTumor Microenvironment Reveal Specific Cellular Niches that DrivePoor Outcome in ER- Breast Cancer
12:00 – 12:45 PM Giuseppe Filomeni, Ph.D. – The Role of SNO Signaling in Cancer
12:45 – 1:30 PM Jack Lancaster – Unresolved Questions in the NO Field
1:00 – 1:30 PM 2028 Meeting Announcement & Closing Remarks by Nathan Bryan
